Data da gravação: June 08 2010

Duration: 58 minutes 31 seconds

Light scattering and chromatography are key tools for protein formulation and characterization studies providing complementary information on protein stability, aggregation, thermal stability, molecular weight, oligomeric state and structure
Table of contents
1. Qualitative and quantitative analysis of proteins using a combination of light scattering techniques in your workflow
02:18
2. Qualitative and quantitative analysis of proteins using a combination of light scattering techniques in your workflow
00:24
3. Pharmaceutical industry
00:52
4. Pharmaceutical Drug Development Pipeline
01:22
5. Where can light scattering be used?
01:03
6. Complementary techniques
01:11
7. Light Scattering
00:49
8. Static Light Scattering – Molecular Weight
00:58
9. Hardware for Analytical SEC
01:28
10. Analytical SEC Chromatogram
00:48
11. Advanced analytical SEC
00:29
12. Dynamic Light Scattering
01:25
13. Correlation Functions
00:58
14. Size Data
01:53
15. Instruments and Applications
00:39
16. Summary of DLS and SEC – pros and cons
02:01
17. Molecular weight and oligomeric state
01:54
18. Conformational changes
01:30
19. Membrane Protein
01:56
20. Aggregate quantification
01:39
21. Effect of Storage Conditions
01:46
22. Oligomeric States of Thermophile protein using DLS
01:35
23. Protein stabilised by ligand binding
00:31
24. Measuring Mw and A2 by static light scattering
01:28
25. Electrophoretic light scattering (Zeta potential)
01:57
26. Which technique is appropriate?
01:59
27. Aggregate detection
00:39
28. Aggregated BSA experiments
00:24
29. Aggregated BSA - DLS experiments
01:56
30. Aggregated BSA - DLS experiments
02:20
31. Aggregated BSA experiments – SEC LS
01:43
32. Aggregated BSA experiments
00:42
33. Qualitative aggregate detection
01:10
34. Oligomer ratio experiments
00:42
35. Oligomer resolution
01:34
36. Oligomer resolution
01:26
37. Formulation
00:54
38. Formulation
01:05
39. Summary
00:51
40. Conclusions
00:46
41. Contact Information
07:26