Data da gravação: November 21 2019

Duration: 40 minutes 43 seconds

The high-resolution size capabilities of the Zetasizer Ultra have enabled a new assay to rapidly characterize Adeno Associated Virus (AAV) viral concentration. Utilizing Multi Angle Dynamic Light Scattering (MADLS), the Zetasizer Ultra can determine both AAV viral concentration and size in a single measurement that is cuvette-based, rapid, label-free, low volume, and non-destructive. In this talk, we present AAV viral concentration and size results, the MADLS measurement principle, and the effect of sample properties, such as material refractive index and viscosity.

Table of contents
1. The Zetasizer Ultra - A novel assay for measuring Adeno-Associated Virus (AAV)
02:21
2. Agenda
01:01
3. Section 1:Introduction to the Zetasizer Ultra and MADLS
00:19
4. Zetasizer – Introduction and Overview
02:56
5. Zetasizer – Key Technology
01:27
6. Zetasizer Ultra – What's new?
02:11
7. Measurement Principle - Dynamic Light Scattering (DLS)
01:55
8. Limitations of Single Angle DLS
02:01
9. Measurement Principle - Multi Angle Dynamic Light Scattering (MADLS)
01:26
10. Zetasizer Ultra - Particle Concentration
01:16
11. Section 2: Adeno-Associated Virus in Gene Therapy development
00:20
12. Adeno-Associated Virus – Gene Therapy Vector
02:25
13. Adeno-Associated Virus – Structure
00:00
14. Adeno-Associated Virus – Common Titer Assays
00:00
15. Adeno-Associated Virus – Zetasizer Ultra Assays
01:28
16. Section 3: AAV Example Data
00:00
17. Zetasizer Ultra – Example AAV Data
00:26
18. Case Study #1: Application Note
01:06
19. Case Study #1: Application Note
01:00
20. Case Study #1: Application Note
01:13
21. Case Study #2: AAV Thermal Stability Test
01:46
22. Case Study #2: Particle Concentration by MADLS
01:36
23. Case Study #2: In-Process AAV Characterization
02:08
24. Section 4: Building a MADLS Method for AAV
00:11
25. ZS Explorer Software
00:21
26. Particle Concentration – How it Works
02:20
27. Zetasizer Ultra – Method parameters
04:26
28. Zetasizer Ultra – Summary and Conclusions
01:38
29. Questions
00:56
30. Thanks for listening
00:30