Areas of expertise
Staining approaches provided by Concept Life Sciences Histology include:
- Staining dyes
e.g., H&E, Alcian blue, Picrosirius red
- Single and multiplex chromogenic immunohistochemistry (IHC)
e.g., DAB, red, green
- Single and multiplex immunofluorescence (IF)
up to 8-plex (9-colour incl. DAPI)
mRNA detection; up to 4-plex
- Mixed multiplex detection
IHC/IF (protein) + RNAscope (mRNA)
Our use of the latest laboratory equipment, including the Leica BOND RX Research Stainer and Vectra PolarisTM imaging system enables us to fully automate many of our staining approaches enabling us to deliver a robust, reliable, and high-throughput service.
CD8, CD31, Haematoxylin
PanCK, C4d, DAPI
CD68, CD8, PanCK, DAPI
PD-L1, PD-1, CD4, CD8, DAPI
8-plex (9-colour) IF
Concept Life Sciences Histology offers simultaneous imaging of up to 8 markers (proteins) + DAPI (nuclear stain) on a single tissue section. This can provide morphological, pathological, and spatial information of the juxtaposition of different cell types, which can be quantified by image analysis.
Image right: CD3, CD8, CD4, FoxP3, CD68, Ki67, PanCK, DAPI with OpalTM Multiplex Detection Reagents imaged using the Vectra PolarisTM from PerkinElmer (human CRC)
Specialized IHC staining approaches utilized during preclinical and clinical drug discovery research provide valuable insights into the location and positioning of biomarkers (cell types) within tissue.
Our knowledge, expertise and pragmatic approach to antibody selection and validation can facilitate demonstration of novel and challenging biomarkers in situ, providing high-value data.
Image right: Human tonsil - T cells (CD3), Cytotoxic T cells (CD8), Regulatory T cells (FoxP3), Myeloid cells (CD68), Epithelial cells (PanCK), Proliferating cells (Ki67), Nuclei (DAPI)