Datum der Aufzeichnung: 15 November 2012

Duration: 53 minutes 46 seconds

Size exclusion chromatography is a well-established technique for characterization of the size, molecular weight and oligomeric state of proteins. New developments are presented with a focus on calibration methods
Table of contents
1. New Analytical Developments in Protein Size Exclusion Chromatography
01:08
2. New Analytical Developments in Protein Size Exclusion Chromatography
00:41
3. Contents
01:19
4. INTRODUCTION TO SEC
00:04
5. Gel Permeation and Size Exclusion Chromatography
01:10
6. Hardware Schematic SEC System
02:42
7. SEC Tetra Detection
01:58
8. Light Scattering
00:41
9. The Principle – Light Scattering
00:30
10. Angular Dependence
01:45
11. Isotropic Scatterers
01:08
12. Viscosity Detector
01:21
13. Relation Between Structure and Intrinsic Viscosity
01:25
14. WHAT IS CALIBRATION?
00:20
15. What is Calibration?
01:56
16. Calibration of Light Scattering Detector
01:15
17. Principles of light scattering
00:52
18. Is light scattering ‘absolute’?
01:19
19. Alternative Calibration Methods
01:41
20. CALIBRATION OF A POLYMER
00:12
21. Calibration of a Polymer in SEC
00:49
22. Calibration of a Polymer in SEC
01:04
23. CALIBRATION OF PROTEINS
00:05
24. Calibration of Proteins in SEC
03:25
25. Current Calibration Standard
01:26
26. Current Calibration Standard
00:38
27. Current Calibration Standard
00:12
28. Current Calibration Standard
00:28
29. Current Calibration Standard
00:13
30. Current Calibration Standard
01:05
31. Current Calibration Standard
01:27
32. Concept
00:37
33. Methods
00:57
34. Methods
02:11
35. Methods
02:01
36. Methods
02:51
37. Methods
04:19
38. Example Calibration Standard - Lysozyme
00:25
39. Example Calibration Standard - Lysozyme
01:55
40. Contact Information
04:11