The NIS assay measures the uptake of iodide into rat FRTL-5 cells in the presence and absence of test item.
The NIS is a co-transporter that mediates uptake of iodide into follicular cells of the thyroid gland as the first step in the synthesis of thyroid hormone. This assay is a spectrophotometric method run with a positive control (and negative control if requested by client). Cell viability is also checked to enable accurate interpretation of results.
Deliverable: Study report detailing protocol, data and IC50 of test compound.