The Kinetics Guide | Binding kinetics with the WAVE system. Download now

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No-clog microfluidics

By combining the patented Grating-Coupled Interferometry (GCI) technology with no-clog microfluidics, the Creoptix® WAVEsystem deliver high quality data from even the most challenging sample types and achieve superior resolution in signal and time compared to other forms of label-free detection. No-clog microfluidics accommodates of a broad range of sample types to preserve activity and biological context, saving time from detrimental purification steps and clogging that takes other systems offline, including 100% whole blood or undiluted serum and plasma, cell supernatant, cell membrane preps, virus-like particles (VLPs), liposomes, viscous detergents, DMSO and acetonitrile.

Clog-free Microfluidics and the Creoptix WAVE system

Thanks to its clog-free microfluidics, Creoptix WAVE offers robustness of crude samples normally only achieved with plate-based assays. The innovative design of the patented microfluidic cartridge, the WAVEchips, supports crude samples, pathogenic samples, harsh solvents and large particles of up to 1000 nm, enabling reliable kinetic analysis. This is made possible by the innovative fluidics design, which integrates all the microfluidics into the disposable cartridge (not within the device core) and places large bore standard valves downstream in the instrument instead of having microvalves near the chip. The cartridge design allows ultra-fast transition times of 150 ms for reliable determination of off-rates of 10 s-1, enabling the kinetic study of weakly binding fragments. 

[Microfluidics - wavechip section view.jpg] Microfluidics - wavechip section view.jpg

Section view of WAVEchip, showing microfluidics integrated into the disposable cartridge by placing the downstream valves instead of microvalves near the chip.

Creoptix WAVEchips cartridge design

The cartridges contain four flow cells in parallel. Typically, one flow cell is used as a reference to account for bulk effects with either no ligand or a reference ligand immobilized. The reference channel can be freely configured on the four-channel WAVEdelta system. While the two-channel WAVE only allows the usage of two flow cells (FC) per experiment (FC1 and FC4; FC2 and FC3), the choice for the reference channel is still free.

[Microfluidics - Wavechip flow configuration.jpg] Microfluidics - Wavechip flow configuration.jpg

Compatibility

The non-clog technology renders the Creoptix WAVEchips compatible with:

  • 100% serum, plasma and cell supernatant
  • Organic solvents, including high percentages of acetonitrile and dimethyl sulfoxide (DMSO)
  • Viscous detergents and additives to solubilize membrane proteins
  • Cell membrane preps, partially solubilized, unpurified material
  • Virus-like particles (VLPs), liposomes, or nanodiscs used as solubilization structures
  • Large binding partners: nanoparticles and crude membrane preps