Protein stability is important in numerous disciplines, ranging from basic and medical biochemistry to pharmaceutical sciences. However, it is rare that researchers from all of the relevant areas can join together to discuss the critical issues in the field. Since 1994 the Colorado Protein Stability Conferences have provided unique opportunities for this exchange of information. The topics to be covered include for the 25th Anniversary Conference in 2019 include: protein folding, dynamics, stability and self assembly; amyloidosis and protein aggregation; and stability, formulation, analysis and processing of therapeutic proteins.