Fecha registrada: February 01 2011

Duration: 01 hours 11 minutes 47 seconds

A presentation of the fundamentals of GPC/SEC, the key performance parameters and the data that can be obtained. Understanding these basic topics will enable the proper columns and chromatographic conditions to be chosen
Table of contents
1. Understanding Size-exclusion Chromatography Basics: Choosing the Correct Columns and Detectors
02:54
2. Understanding Size-exclusion Chromatography Basics: Choosing the Correct Columns and Detectors
01:37
3. Outline
01:21
4. Defining Some Terms
03:28
5. Why Use Size-exclusion Chromatography?
03:21
6. Sample Solubility Required
01:56
7. Components of an SEC System
01:11
8. The Separation Process
03:38
9. Plate Theory
02:40
10. Flow-rate Effects – van-Deemter
03:57
11. SEC Chromatogram – Gaussian Peak
02:06
12. Deviation from Ideal Peak Shape
02:29
13. Column Range
02:22
14. Column Resolution
02:05
15. Calibration Slope
02:48
16. Selecting a GPC/SEC Column
04:12
17. Common Mobile Phases – General Guidelines
00:51
18. Why Are Salts and Buffers Used?
01:54
19. SEC Performance Factors Summary
01:46
20. SEC/GPC Data Processing Options
01:47
21. Column Calibration Configurations
01:55
22. Absolute Detection Configurations
01:26
23. Making the Final Decision
02:03
24. Always Remember
01:29
25. Conclusions
01:29
26. Defining Some Terms
12:14
27. Contact Information
01:24
28. Contact Information
01:24