Fecha registrada: June 14 2011

Duration: 27 minutes 48 seconds

The Zetasizer Nano uses a new diffusion barrier method to protect proteins during a measurement of their charge. The presentation outlines the method and presents a number of application examples
Table of contents
1. Protein zeta potential measurements using Malvern’s new diffusion barrier method
02:10
2. Protein zeta potential measurements using Malvern’s new diffusion barrier method
00:12
3. Outline
00:31
4. Zeta Potential (ZP)
01:25
5. Measuring Zeta Potential
00:51
6. Zeta potential measurements using micro-electrophoresis
01:05
7. Laser Doppler Electrophoresis
00:58
8. Measurement strategy (M3-FFR)
01:43
9. Henry’s Equation
00:40
10. Zeta potential measurements of proteins
00:35
11. Diffusion barrier technique
02:05
12. Diffusion barrier technique
01:39
13. HSA measurement – full cell
01:26
14. HSA measurement – diffusion barrier
00:40
15. Size distributions before and after measurements
00:43
16. Important parameters for protein zeta potential
00:55
17. Investigating MEP using BSA in PBS+1mM DTT(4) Polarisation concentration effects
01:30
18. Investigating MEP using BSA in PBS+1mM DTT(5)
01:34
19. HSA in different pH buffers
00:18
20. HSA sample measured with diffusion barrier
00:41
21. General recommendations for MEP/zeta potential of protein samples in folded capillary cell
01:40
22. Conclusions
00:48
23. Thanks for listening
03:04
24. Contact Information
00:35