The microcalorimetry of nanoparticles

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00:00:00 Welcome
00:00:09 Welcome
00:02:02 Microcalorimetry of nanoparticles
00:02:18 Outline
00:03:31 Nanotechnology and nanoparticles
00:04:22 Nanotechnology and nanoparticles
00:04:54 Nanotechnology and nanoparticles
00:06:43 Nanoparticles in biomedical research
00:08:13 Overview of nanoparticles and applications in life sciences/biomedicine
00:08:48 Design of nanoparticles for drug delivery
00:10:09 Analytical methods to characterize nanoparticles
00:11:12 Why microcalorimetry for characterization of nanoparticles and biomolecules?
00:13:51 Microcalorimetry systems from Malvern Instruments
00:14:23 What is Isothermal Titration Calorimetry (ITC)?
00:15:35 Performing an ITC assay
00:17:27 Basics of ITC experiment
00:20:15 The energetics
00:21:41 ITC characterizes a broad range of interactions
00:22:02 Isothermal titration calorimetry and nanoparticles
00:23:13 How ITC is used in nanoparticle characterization
00:24:06 Isothermal Titration Calorimetry Studies on the Binding of Amino Acids to GoldNanoparticles
00:25:14 Binding of aspartic acid to gold nanoparticles
00:27:38 Binding of lysine to gold nanoparticles
00:29:01 Conclusions
00:29:37 Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles
00:30:42 ITC of HSA and nanoparticles
00:32:02 Conclusions
00:32:42 Trapping RNase A on MCM41 pores: effects on structure stability, product inhibition and overall enzymatic activity
00:34:07 ITC of absorption of RNase on MCM41
00:34:57 Enzyme catalysis by ITC – RNase in absence and presence of MCM41
00:37:24 Conclusions
00:37:59 Elucidating the molecular mechanism of PAMAM–siRNA dendriplex self-assembly: Effect of dendrimer charge density
00:38:46 ITC of siRNA with dendrimers
00:40:15 Conclusions
00:41:03 Enhanced Antitumor Activity of the Photosensitizer meso-Tetra(N‑methyl-4-pyridyl) Porphine Tetra Tosylate through Encapsulation in Antibody Targeted Chitosan/Alginate Nanoparticles
00:42:09 ITC of TMP and alginate
00:43:00 Conclusions
00:43:14 Formation of nanoparticles by cooperative inclusion between (S)-camptothecin-modified dextrans and β-cyclodextrin polymers
00:44:44 Formation of polymer-β-CD complex by ITC
00:45:27 Conclusions
00:46:09 Peptide-Mediated Constructs of Quantum Dot Nanocomposites for Enzymatic Control of Nonradiative Energy Transfer
00:47:16 ITC of polyelectrolyte peptides
00:47:54 ITC data of peptide pairs
00:48:39 Conclusions
00:48:58 Isothermal titration calorimetry and nanoparticles
00:49:49 MicroCal VP-ITC system from Malvern Instruments
00:50:32 MicroCal iTC200 system from Malvern Instruments
00:51:13 Thank you!!
00:57:00 The energetics
00:59:42 Contact Information
01:01:17 Contact Information
01:01:26 More information

In this webinar, find out how Isothermal Titration Calorimetry (ITC) can be used to characterize nanoparticles. Isothermal titration calorimetry (ITC) is used to characterize the thermodynamics of nanoparticle assembly, as well as nanoparticle interactions with proteins, membranes and other molecules. ITC is label free and can be used with nanoparticles in suspension.