Determination of protein solution viscosity using DLS microrheology

An application note to demonstrate how Dynamic Light Scattering (DLS) based microrheology can be used to accurately measure the complex viscosity of protein solutions.The viscosity of bovine serum albumin BSA in phosphate buffered saline prepared between 10 mg/mL and up to 400 mg/mL was determined with carboxylated melamine microspheres MEL0665 . The method uses the Generalized Stokes-Einstein Relation to find the mean square displacement MSD and complex viscosity.

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