記録された日時: April 01 2010

Duration: 44 minutes 02 seconds

There are a number of calibration techniques using protein standards for measuring protein molecular weight, the most common being conventional calibration. Why should you upgrade to add light scattering and viscosity detectors?
Table of contents
1. Protein applications for tetra detection. Why upgrade from calibration against protein standards?
01:37
2. The Benefits of Tetra Detection for Protein Characterisation
01:05
3. GPC or SEC separates macromolecules in solution
02:01
4. Conventional Calibration
02:58
5. Limitations of conventional SEC
01:06
6. Less compact protein
01:57
7. Non-globular protein
01:28
8. Proteasome 20S filled with Cytochrome C
01:58
9. Fractionation
01:36
10. Hardware for Tetra Detection
01:19
11. Tetra Detection
00:58
12. UV Detection
00:31
13. RI Detection
00:59
14. Light Scattering detection
01:44
15. RALS/LALS Combination
03:52
16. Intrinsic Viscosity 4- capillary Differential Viscosity Detector
02:04
17. Typical Tetra Detector Chromatogram
01:31
18. Tetra Detection
00:26
19. Molecular weight measurements
00:27
20. Standard Protein – Bovine Serum Albumin
01:56
21. Standard Protein – Bovine Serum Albumin
00:09
22. Oligomer determination
01:08
23. Proteasome 20S filled with Cytochrome C
00:20
24. Protein Aggregation
00:27
25. Cases
00:19
26. FDA & USP
00:17
27. Aggregate quantification
01:33
28. Conformational changes
00:29
29. Conformational changes
01:55
30. Antibody analysis
00:23
31. Protein Conjugation
00:49
32. Co-polymer analysis
00:44
33. PEGylated Protein
01:00
34. Conjugation analysis
00:50
35. Conjugation analysis
00:14
36. Summary
00:57
37. Contact Information
00:55