International Symposium on GPC/SEC and Related Techniques

Malvern is sponsoring the International Symposium on GPC/SEC and Related Techniques. It is a unique event on the scientific calendar which focuses on bringing together the three key elements of Instrumentation Innovation, Industrial Research and Academia. Through a series of scientific presentations and open forum discussions, led by some of the most renowned experts in this field, attendees are immediately immersed into open debate surrounding the most important and current analytical challenges facing the polymer characterization industry.

Wednesday, September 28, 2016 - 3:30 - 3:50
John Stenson will give a talk focusing on "Combining multi-detector GPC measurements with ultra-high pressure GPC separations"

We really look forward to seeing you there!


John Stenson, Technical Support Supervisor

Combining multi-detector GPC measurements with ultra-high pressure GPC separations

Abstract:  Gel-permeation chromatography (GPC) is the most widely used tool for the measurement of molecular weight and molecular weight distribution of polymers.  In recent years, Ultra High Pressure Chromatography (UHPLC or UPLC™) has advanced into GPC separation offering clear benefits including improved resolution, as well as reduced sample requirement, solvent usage, and run times, all of which are highly desirable.

Multi-detector GPC measurements include light scattering detectors and viscometers to measure molecular weight, intrinsic viscosity, Mark-Houwink parameters and other related molecular information.  Molecular weight from light scattering is a direct measurement independent of sample retention volume.

Combining multi-detection and UPLC™ is a challenging proposition due to the increased impact of band-broadening and dispersion that occurs as the sample moves between detector cells and through the inter-detector tubing.  

This presentation will discuss the issues related to dispersion and how this can be minimized.  We will then show the first high quality data from a combined multi-detector/ UPLC™ system comprising a Waters APC system with Malvern’s OMNISEC REVEAL multi-detector module.  The measurements will show that despite some loss of resolution between the detectors it is still possible to make high quality multi-detector measurements of polymers and proteins separated under UPLC™ conditions.  Absolute molecular weight and intrinsic viscosity measurements are successfully made thanks to the minimization of dispersion between detector cells.



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