Зафиксированная дата: November 12 2015

Duration: 46 minutes 25 seconds

Isothermal Titration Calorimetry (ITC) is a technique that measures the heat released or absorbed during a chemical reaction as an intrinsic probe to characterize any chemical process. In the present webinar, the experimental ITC-based methods to quantify kinetics and thermodynamics of enzymatic reactions are described.

ITC can concomitantly determine kinetics and thermodynamic parameters (kcat, KM, DH) of enzymatic reactions, not requiring any modification or labelling of the system under analysis, as heat changes spontaneously during enzymatic catalysis.

ITC can be performed in solution and needs little amount of material. These properties make this technique an invaluable, powerful and unique tool to extend the study of enzyme kinetics to several applications, such as drug discovery.
Table of contents
1. Welcome
01:23
2. Calorimetry- Basics
01:48
3. Isothermal Titration Calorimeter
01:44
4. Enzyme Kinetics
00:57
5. Enzyme Kinetics
01:49
6. Enzyme Kinetics and ITC
01:32
7. Determination of Enthalpy- M1
00:59
8. Experimental Considerations- M1
00:49
9. Calculating "True" Reaction Enthalpy
01:30
10. Determining dQ/dt
01:10
11. Experimental Considerations- M2
01:04
12. Determining dQ/dt
00:22
13. Continuous assay
00:33
14. Continuous assay
01:02
15. Experimental Considerations- Single Injection Method
01:19
16. Practical Considerations
01:39
17. Ni(ll)- Dependent Urease
01:01
18. Ni(ll)- Dependent Urease
00:29
19. Ni(ll)- Dependent Urease
01:02
20. Lactate Dehydrogenase
00:47
21. Lactate Dehydrogenase
00:23
22. Laccase
01:08
23. Enzyme Inhibition by ITC
01:35
24. Enzyme Inhibition by ITC
00:22
25. Urease Inhibition by Fluoride
01:56
26. LDH-A Inhibition by Oxamate
01:08
27. Trypsin Inhibition by Benzamidine
00:43
28. Conclusions
00:46
29. Acknowledgments
00:16
30. Thank you for your attentionAny questions?
14:32
31. Contact Information
00:37