Quality control of purified proteins: a critical key to success

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Purified protein quality control is the final critical check-point of any protein production process. Unfortunately, it is too often overlooked altogether or performed hastily, resulting in a lot of time wasted on poor quality samples, confirming the adage ‘garbage in, garbage out’. Sample quality can be improved by convincing scientists to perform quality control as the final critical check-point of their protein production process.

The Association of Resources for Biophysical Research in Europe (ARBRE-MOBIEU) and the Protein Production and Purification Partnership in Europe (P4EU) have teamed up to propose an easy-to-follow workflow based on an ensemble of widely available physicochemical technologies. This workflow enables sequential assessment of the essential properties of any protein sample: purity and integrity, homogeneity and activity. Approaches are suggested to optimize the homogeneity, time-stability and storage conditions of purified protein preparations, as well as methods to rapidly evaluate their reproducibility and lot-to-lot consistency. The workflow and results obtained in our laboratory will be presented in this webinar, and its clear time and money savings will be discussed.

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Bertrand Raynal - Institut Pasteur, Molecular Biophysics Platform - Paris, France

Bertrand earned his MSc in biochemistry at the UPS (Toulouse), and completed his PhD and post-doctorate in the Wellcome Trust Centre for Cell-Matrix Research in Manchester, UK.

Bertrand has worked as an experimental officer in the biophysical core facility within the Institut Pasteur (PFBMI) since 2007. He leads the hydrodynamic characterization area (AUC, SLS viscometry, DLS, DPI, SAXS) and is also responsible for the Institut’s services for quality control of purified proteins.

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Why will you learn?

How to better use quality control assessment strategies within protein production in order to identify and control issues earlier in the pipeline. Attendees will also understand how a recently-developed physicochemical workflow is being used to fully assess and optimize protein quality, stability and reproducibility.

Who should attend?

Scientists working within the field of protein manufacture, especially purification, and those interested in quality control workflows.