Questions we can help you answer
How do I identify potential therapeutic targets in my disease of interest?
We can support your understanding of disease pathology by providing analysis of matched tissue between diseased and non-diseased patients. We can interrogate pathways, genes and protein targets involved by performing expression profiling (e.g. taqman, IHC, Western, RNA-Seq, Nanostring) as well as developing novel custom primary cell and co-culture assays to explore your hypothesis.
How can I understand if the phenotype I need to inhibit is due to the enzyme activity or the scaffolding function of my protein?
We provide a Targeted protein degradation service. We can design PROTAC degraders to eliminate your protein of interest at the protein level and interrogate the phenotype of protein knockdown. We can also identify novel inhibitors via our unique biophysical fragment-based drug discovery platform.
What is my drug’s mode of action?
We design custom novel biophysical and biochemical assays to determine the binding mode and mechanism of protein-drug interaction. For example, we can interrogate whether your drug candidate prevents a protein complex associating, or stabilizes a protein complex in an inactive state thus inhibiting downstream events. We can also interrogate the kinetic profiles of these interactions. Or we can look at whether your compound is an activator, or inhibitor, (competitive, non-competitive), whether your binding mode is allosteric or orthosteric.
What are the downstream targets and biological consequences of the action of my drug?
We can support assay design in cell-based assays to provide analysis of the molecular signaling pathways impacting in different cell and tissue types.
Are the results I see in in vitro assays mirrored in vivo and in disease-relevant states?
We also support these models with a range of ex vivo analyses such as brain slice models, multiplex histology, Nanostring and RNA-seq
How do I know that the results I see in my in vivo model will translate to the clinic?
We are experts in developing human in vitro cell-based co-culture disease models using primary cells, tissue (diseased and non-diseased) or cell lines. We also provide matched models and readouts in rodent in vitro models to support the translation of your in vivo efficacy studies to prediction of drug behavior in humans.
An output of this analysis is that we identify potential biomarkers and/or bioanalytical readouts suitable for monitoring outcomes in your clinical trial (eg qPCR, LCMS/MS, MSD, Flow cytometry, Histology, RNA-seq and ELISA readouts)