A basic introduction to Dynamic Light Scattering (DLS) for particle size analysis

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00:00:00 Welcome
00:00:14 Introduction
00:00:19 Dynamic light scattering in 30 Minutes
00:00:55 Agenda
00:01:12 Agenda
00:01:15 Dynamic Light Scattering and Brownian Motion
00:02:31 Brownian Motion
00:03:22 Hydrodynamic Size
00:05:13 DLS Instrument Components
00:07:35 Intensity Fluctuations and Brownian Motion
00:08:11 Constructive and Destructive Interference
00:09:58 Agenda
00:10:03 Correlation in Dynamic Light Scattering
00:11:59 Correlation
00:12:44 Correlation
00:14:31 Correlation
00:15:15 Correlation
00:15:44 Correlation
00:15:49 Correlation
00:16:56 Correlation
00:18:01 Correlation Functions
00:18:12 Agenda
00:18:34 Correlation Functions
00:19:43 Analysing The Correlation Function
00:19:48 Analysing The Correlation Function
00:20:55 Cumulants Analysis
00:22:21 The z-Average Diameter
00:23:11 Polydispersity Index
00:24:27 Zetasizer Distribution Algorithms
00:27:18 Size Distributions in the Zetasizer Software
00:28:22 Intensity Size Distributions
00:29:49 Volume Size Distributions
00:31:18 Number Size Distributions
00:32:06 Size Distributions From DLS
00:32:54 Volume/Number Distributions: Recommended Use
00:33:46 Any Questions?

Dynamic Light Scattering (DLS), sometimes referred to as Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering, is a technique classically used for measuring the size of particles typically in the sub-micron region, dispersed in a liquid. The sensitivity of some modern systems is such that it can also now be used to measure the size of macromolecules in solution.

In this presentation Dr Mike Kaszuba will summarize how the technique works, what its size and concentration limits are, and discuss its advantages and disadvantages.


Dr Mike Kaszuba: Michael Kaszuba is Technical Support Manager at Malvern Instruments Ltd. He has a PhD from the Polytechnic of Wales, where he studied the physical biochemistry of liposomes using nuclear magnetic resonance techniques. He followed this with postdoctoral research into liposomal drug delivery at the University of Manchester. Michael joined Malvern in 1996 as a Product Technical Specialist and two years later was appointed in his current role, responsible for the Product Technical Specialist and Application groups based in the UK.

Mais informações

Who should attend?
Anyone who is working with or interested in Dynamic Light scattering and would like an overview of the technique including its benefits and limitations.

What will you learn?
About how Dynamic Light Scattering works, including the generation and interpretation of the correlation function and how this is used to give particle size information.

Why attend?
This is an opportunity to get a basic overview of Dynamic Light Scattering and its applications from one of Malvern Panalytical's Zetasizer experts.