Creoptix - Discovery of CLIPS™ binders to anti-TNFα mAb's using Phage Display Libraries

Mimotope peptides selected from combinatorial peptide libraries can be used as capture reagents for immunoassay detection of therapeutic monoclonal antibodies (mAbs). Previous attempts by Abreos, independent of Pepscan, to identify peptide-binders from 7aa and 12aa linear peptides and C7C and 7C7C7 SS-looped peptide libraries did not yield any hits that showed reproducible binding in ELISA. Here we report the use of a monocyclic phage library encoding for a fully randomized 10-mer peptide sequence flanked by two cysteines that are linked via a CLIPSTM scaffold (ACT2XXXXXXXXXXCT2G) to identify peptide binders (VeritopesTM) that bind to Infliximab (brand name RemicadeTM), a therapeutic mAb indicated to treat autoimmune diseases like Rheumatoid Arthritis (RA) and Crohn’s Disease.

Mimotope peptides selected from combinatorial peptide libraries can be used as capture reagents for immunoassay detection of therapeutic monoclonal antibodies (mAbs). Previous attempts by Abreos, independent of Pepscan, to identify peptide-binders from 7aa and 12aa linear peptides and C7C and 7C7C7 SS-looped peptide libraries did not yield any hits that showed reproducible binding in ELISA. Here we report the use of a monocyclic phage library encoding for a fully randomized 10-mer peptide sequence flanked by two cysteines that are linked via a CLIPSTM scaffold (ACT2XXXXXXXXXXCT2G) to identify peptide binders (VeritopesTM) that bind to Infliximab (brand name RemicadeTM), a therapeutic mAb indicated to treat autoimmune diseases like Rheumatoid Arthritis (RA) and Crohn’s Disease.

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