Augmented affinity in antibody-antigen interactions

To investigate the binding dynamics of the antibody pair in this assay, mAb1 (sheep) and mAb2 (mouse) the binding kinetics were measured in the Creoptix WAVEdelta system. Four reagent combinations were interrogated in real time either as p24 antigen to each immobilized antibody or a p24/antibody complex binding to the second antibody, both immobilized and free in solution

The results reveal a surprising enhancement in affinity due to a considerable on rate shift when mAb1 recognizes the mAb2 p24 complex.

To investigate the binding dynamics of the antibody pair in this assay, mAb1 (sheep) and mAb2 (mouse) the binding kinetics were measured in the Creoptix WAVEdelta system. Four reagent combinations were interrogated in real time either as p24 antigen to each immobilized antibody or a p24/antibody complex binding to the second antibody, both immobilized and free in solution

The results reveal a surprising enhancement in affinity due to a considerable on rate shift when mAb1 recognizes the mAb2 p24 complex.

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