MicroCal label-free interaction analysis
MicroCal VP-ITC is a sensitive isothermal titration calorimeter that is easy to use and operated using a software interface for fast and accurate analysis.
MicroCal VP-ITC applications include characterization of molecular interactions of proteins, antibodies, nucleic acids, lipids, and other biomolecules. It is widely used in industry for lead optimization, hit validation, assessment of the effect of molecular structure changes on binding mechanisms, and enzyme kinetics.
MicroCal VP-ITC delivers:
- Affinity data that provides insights into biomolecular interactions
- The ability to investigate any biomolecular interaction
- Convenience and speed since there is no need for immobilization and labeling
- Free choice of buffers and no limitations with respect to molecular weight
- Unattended operation after sample loading, allowing you to focus on other tasks
- A complete system without the need for additional accessories, reagents or consumables
Isothermal titration calorimetry (ITC) is a powerful analytical tool that measures the binding affinity and thermodynamics between any two biomolecules.
When substances bind, heat is either generated or absorbed. Measurement of this heat change in an ITC experiment enables accurate determination of binding affinity (KB), reaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment.
MicroCal VP-ITC can also be used to determine heat capacity changes upon binding by performing experiments over a range of temperatures.
Figure 1: MicroCal VP-ITC.
MicroCal VP-ITC features:
Directly measures millimolar to nanomolar binding constants (102 to 109 M-1)
Measures nanomolar to picomolar binding constants using competitive binding techniques (109 to 1012 M-1)
Nonreactive Hastelloy™ cells for excellent chemical resistance
Fixed-in-place cells for reproducible, ultrasensitive performance with low maintenance
Precision liquid delivery system for accurate and reproducible injections
Three user selectable response times for application versatility
User-selectable mixing speeds to match sample conditions
Peltier controlled for rapid temperature equilibration
Includes ThermoVac sample preparation and cleaning device
Controlled by VPViewer software and data analysis performed with Origin
|Operating temperature range||2° to 80°C|
|Response time||20 s|
|Cell design||Coin-shaped, fixed in place, non-removable, non-reactive|
|Cell volume||1400 μl (1800 μl to fill)|
|Injection syringe volume||300 μl|
|Sample throughput||4-8 per 8 hour day|
|Dimensions (W × H × D)||20.3 × 43.8 × 36.5 cm|