Fecha registrada: June 21 2012

Duration: 01 hours 04 minutes 52 seconds

This webinar gives examples of the complementary nature of dynamic light scattering for fast aggregate detection and size exclusion chromatography for the composition and molecular weight characterization of proteins
Table of contents
1. Speed vs. Detail: Dynamic light scattering (DLS) vs size exclusion chromatography (SEC)
01:25
2. Speed vs. Detail: Dynamic light scattering (DLS) vs size-exclusion chromatography (SEC)
00:54
3. Outline
00:44
4. Introduction to DLS and (multi-detector)-SEC
00:08
5. Dynamic Light Scattering
02:57
6. Size Data
03:20
7. Hardware for Analytical SEC
01:34
8. Multi-detector-SEC
03:12
9. So what?
00:20
10. What can DLS do?
00:21
11. Effect of Storage Conditions
04:19
12. Purity – Polydispersity
01:22
13. What can DLS do?
00:19
14. Protein stabilised by ligand binding
02:23
15. What can DLS do?
00:26
16. Protein Stability
03:25
17. What can DLS do?
00:23
18. Formulation
02:51
19. What can DLS do?
00:38
20. Limitations of DLS
01:40
21. What can SEC do?
00:09
22. Molecular weight measurements of BSA
02:35
23. Resolution
01:55
24. What can SEC do?
00:11
25. Quantification of antibody aggregates
02:05
26. What can SEC do?
00:15
27. Composition – Membrane proteins
01:45
28. Composition – Membrane proteins
02:26
29. PEGylated Protein
00:37
30. Conjugation analysis
01:00
31. What can SEC do?
00:17
32. Conformational changes
03:06
33. What can SEC do?
00:14
34. Limitations of SEC
01:37
35. Summary of DLS and SEC – pros and cons
01:22
36. Summary
00:33
37. So the real question is…..
00:11
38. So the real question is…..
00:08
39. Thanks for listening Any questions?
00:15
40. Contact details
11:30