Fecha registrada: June 27 2017

Duration: 39 minutes 54 seconds

Membrane protein research is dependent on the availability of membrane mimics that retain the structure and function of the target protein within a lipid-bilayer environment while rendering it amenable to in vitro investigations.

Styrene/maleic acid (SMA) copolymers extract phospholipids and proteins from artificial and natural membranes to form polymer-bounded nanodiscs (SMALPs) which retain the lipid-bilayer architecture of the host membrane. Therefore, SMALPs are gaining increasing interest among membrane-protein researchers as an attractive alternative to conventional membrane mimics such as micelles and liposomes. However, the morphology of SMALPs, and, in particular, the extent to which they provide a “protein-friendly” lipid-bilayer environment, remains poorly characterized.

In this webinar, we will demonstrate how multi-detector GPC/SEC provides compositional and morphological information on SMALPs, contributing to a better, quantitative understanding of these new membrane-mimetic nanoparticles.

Table of contents
1. Welcome
00:13
2. Introduction
00:45
3. Revealing the secrets of nanodisc architecture using multi-detection GPC/SEC
00:13
4. Outline
01:08
5. Motivation
01:17
6. Motivation
00:20
7. Motivation
01:47
8. Motivation
01:42
9. Theory: Omnisec
00:09
10. OmniSEC
00:54
11. Untitled
00:46
12. Concentration detectors(RI and UV absorption)
01:33
13. Molar mass detector(static light scattering)
02:10
14. Molecular size detector(viscometry)
01:24
15. Molecular size detector(dynamic light scattering)
00:50
16. Results: BSA and SMALPs
00:13
17. Experimental conditions
01:26
18. BSA chromatogram
00:51
19. BSA analysis
01:08
20. BSA analysis
00:38
21. SMALP analysis(method)
00:56
22. SMALP chromatogram
02:13
23. SMALP analysis (SMA distribution)
01:05
24. SMALP analysis (SMA distribution)
01:06
25. SMALP analysis (SMA distribution)
01:00
26. SMALP analysis (SMALP and SMA molar mass)
00:38
27. SMALP analysis (SMALP size and composition)
01:45
28. SMALP analysis (SMALP size and shape)
02:50
29. Summary and conclusions
01:05
30. Acknowledgements
00:30
31. Q&A
06:37
32. Contact Information
00:42