Fecha registrada: July 25 2017

Duration: 26 minutes 39 seconds

Nanoparticle Tracking Analysis (NTA) is a particle characterization technique that has grown rapidly since its introduction to the market ten years ago. The current NanoSight instruments have become a critical tool used in many applications to provide information not available by other methods. The ability to visualize individual particles for analysis provides single-particle resolution as well as a direct measure of concentration. The technique also works under fluorescence, allowing speciation of labeled populations in complex backgrounds.

This webinar will review the basic principles of the technique itself, compare it to other established techniques like Dynamic Light Scattering, and provide some example application data sets to show how the information is used in practice.

Table of contents
1. Welcome
00:10
2. Introduction
00:41
3. Basic Principles of Nanoparticle Tracking Analysis (NTA)
00:25
4. Outline of Presentation
00:36
5. Seeing is Believing!
00:00
6. Patented Optical Arrangement
01:57
7. NTA Experimental Protocol
00:54
8. Particle Sizing in action - Software Analysis
00:00
9. Sizing: Stokes-Einstein
00:00
10. Concentration: Quantify particles in known volume
00:00
11. Concentration of selected range
00:17
12. NTA Specifications: Size and Concentration Limits
02:04
13. Fluorescence measurement (optional)
00:00
14. Analysis in Complex Biological Media
00:00
15. Available options for fluorescence
00:29
16. Flow Mode for better reproducibility
01:13
17. Industry & Scientific Community Acceptance
01:19
18. Parameters measured by NTA
00:07
19. Size with high resolution
00:00
20. Distribution – shape, breadth, modes
01:19
21. Concentration (Number Base)
00:00
22. Concentration – tracking aggregation
00:00
23. Concentration – protein aggregation
00:52
24. Fluorescence speciation
00:00
25. Relative Light Intensity
00:59
26. DLS and NTA
00:36
27. Getting the same answer
00:36
28. Biological Vesicles and a smaller population
00:44
29. Antibody-functionalized gold nanoparticles
00:37
30. Comparison: NanoSight, DLS, TEM
00:23
31. Correlation: NanoSight, plaque assay, qPCR
00:44
32. Application examples
00:02
33. NTA is applicable to many sample types:
00:21
34. Purified Adeno Virus
00:28
35. Monitoring Virus Purification - Influenza
00:23
36. Exosome Size Distribution Comparison
00:00
37. Aqueous colloidal dispersions
00:00
38. Metals, metal oxides dispersions
00:00
39. Magnetic Nanoparticles
00:46
40. Nanobubbles
00:00
41. Thank you for your time.Questions?
07:32
42. Contact Information
00:05