From the Applications Laboratory:What can GPC do for me?

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00:00:00 From the applications laboratory What can GPC do for me?
00:02:03 From The Application Lab – What Can GPC Do For Me?
00:02:23 Abstract
00:03:04 Outline
00:04:01 Defining Some Terms
00:05:01 Defining Some Terms
00:06:39 What is Chromatography?
00:07:26 Size Exclusion Chromatography
00:09:10 The Separation Process
00:10:46 Why Use Size Exclusion Chromatography?
00:12:37 Molecular Weight Moments
00:13:25 Molecular Weight Moments – Distribution Averages
00:14:19 Understanding Your Sample
00:16:22 Components of an SEC System
00:18:23 Components of an SEC System
00:18:32 SEC/GPC Data Processing Options
00:19:29 Conventional Calibration
00:19:39 Conventional Calibration – Detectors from Viscotek
00:20:42 Concentration Detectors
00:21:17 Creating a Calibration Curve
00:22:07 Conventional GPC Systems
00:22:32 Overlay of Conventional Calibration Curves
00:23:18 Conventional Calibration
00:24:13 Conventional Calibration - Summary
00:24:53 Universal Calibration
00:25:12 Four-Capillary viscometer
00:26:03 Universal Calibration
00:27:10 Universal Calibration – PMMA 64 kDa Narrow Standard
00:28:31 Universal Calibration Conclusions
00:29:40 Triple Detection
00:29:55 Static Light Scattering – Rayleigh Theory
00:30:23 Static Light Scattering Summary
00:31:14 Triple Detection
00:33:30 Process Prediction Based on Structure
00:34:54 Long-Chain Branching
00:35:16 How is LCB Determined?
00:36:11 Structural Differences in Cellulose Excipients
00:37:13 Density Changes in CPVC -QA/QC
00:38:06 Applications – Variables To Consider
00:40:04 Aminated Chitosan – Cationic Column Selection
00:40:45 Polyalkylacrylamide - Inert Column Selection
00:41:33 PET in HFIPA – Inert Column Selection
00:42:28 HPMC in Acetic Acid – Glycosylated Column Selection
00:43:26 Polycarbonate
00:45:14 Functionalizing Chitosan
00:46:01 Functionalizing Chitosan
00:46:32 HT-GPC Applications
00:46:37 Polymers For High Temperature GPC/SEC
00:47:12 Polyethylene (PE) and Polypropylene (PP)
00:48:19 PE and PP at 140 °C in TCB
00:49:36 PP at 160 °C in TCB
00:51:29 Protein Analysis
00:51:36 Intrinsic Viscosity for Proteins
00:52:12 Tetra Detection GPC for Proteins - BSA
00:52:47 BSA – Molecular Weight Determination of Complexes
00:53:31 Untitled
00:54:06 BSA Aggregation – Chromatography Temp. = 45 °C
00:54:43 Compositional Analysis
00:55:34 PEGylated Proteins – Compositional Analysis
00:56:08 Untitled
00:56:41 Conclusions
00:58:04 Contact Information
This overview of GPC/SEC gives examples of polyolefins, proteins and biopolymers that benefit from this powerful analytical tool. Applications range from standard macromolecular characterization to advanced triple detection