| 00:00:00 | Welcome |
| 00:00:10 | Introduction |
| 00:00:51 | Basic Principles of Nanoparticle Tracking Analysis (NTA) |
| 00:01:16 | Outline of Presentation |
| 00:01:52 | Seeing is Believing! |
| 00:01:52 | Patented Optical Arrangement |
| 00:03:49 | NTA Experimental Protocol |
| 00:04:43 | Particle Sizing in action - Software Analysis |
| 00:04:43 | Sizing: Stokes-Einstein |
| 00:04:43 | Concentration: Quantify particles in known volume |
| 00:04:43 | Concentration of selected range |
| 00:05:00 | NTA Specifications: Size and Concentration Limits |
| 00:07:04 | Fluorescence measurement (optional) |
| 00:07:04 | Analysis in Complex Biological Media |
| 00:07:04 | Available options for fluorescence |
| 00:07:33 | Flow Mode for better reproducibility |
| 00:08:46 | Industry & Scientific Community Acceptance |
| 00:10:05 | Parameters measured by NTA |
| 00:10:12 | Size with high resolution |
| 00:10:12 | Distribution – shape, breadth, modes |
| 00:11:31 | Concentration (Number Base) |
| 00:11:31 | Concentration – tracking aggregation |
| 00:11:31 | Concentration – protein aggregation |
| 00:12:23 | Fluorescence speciation |
| 00:12:23 | Relative Light Intensity |
| 00:13:22 | DLS and NTA |
| 00:13:58 | Getting the same answer |
| 00:14:34 | Biological Vesicles and a smaller population |
| 00:15:18 | Antibody-functionalized gold nanoparticles |
| 00:15:55 | Comparison: NanoSight, DLS, TEM |
| 00:16:18 | Correlation: NanoSight, plaque assay, qPCR |
| 00:17:02 | Application examples |
| 00:17:04 | NTA is applicable to many sample types: |
| 00:17:25 | Purified Adeno Virus |
| 00:17:53 | Monitoring Virus Purification - Influenza |
| 00:18:16 | Exosome Size Distribution Comparison |
| 00:18:16 | Aqueous colloidal dispersions |
| 00:18:16 | Metals, metal oxides dispersions |
| 00:18:16 | Magnetic Nanoparticles |
| 00:19:02 | Nanobubbles |
| 00:19:02 | Thank you for your time.Questions? |
| 00:26:34 | Contact Information |
Nanoparticle Tracking Analysis (NTA) is a particle characterization technique that has grown rapidly since its introduction to the market ten years ago. The current NanoSight instruments have become a critical tool used in many applications to provide information not available by other methods. The ability to visualize individual particles for analysis provides single-particle resolution as well as a direct measure of concentration. The technique also works under fluorescence, allowing speciation of labeled populations in complex backgrounds.
This webinar will review the basic principles of the technique itself, compare it to other established techniques like Dynamic Light Scattering, and provide some example application data sets to show how the information is used in practice.
presentadores
Duncan Griffiths is Product Manager for the NanoSight product line, responsible for developing and supporting the NTA technique in the American markets. As Bioscience Business Development Specialist, he has additional responsibilities for expanding opportunities in bioscience applications areas. Prior to NanoSight’s acquisition by Malvern, he was Business Development Manager at NanoSight and has many additional years of experience in the particle characterization field.
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Who should attend?
Users of Dynamic Light Scattering instruments interested in complementary information
Nanoparticle technologists looking for higher performance characterization techniques.
Anybody frustrated by the limits of traditional sizing techniques.
What will you learn?
Basic principles of Nanoparticle Tracking Analysis technique.
How NTA compares to traditional particle sizing techniques.
The powerful capabilities and data provided by this unique view of particle dispersions.
Optimization of the technique and analytical design that yield the highest quality data.