The Chromatogram Series: Dextran
This is a screenshot of the OmniSEC profile of dextran. We offer a set of broad Dextran standards for light scattering and triple detection. The run conditions are detailed in the table below.
| Run Buffer | PBS |
| Flow Rate [mL/min] | 0.700 |
| Column Set | 2 x A6000M |
| Column Temp (°C) | 35.0 |
| Injection volume μL | 100.0 |
| Concentration [mg/mL] | 2.97 |
| Mw Weight Average [Da] | 73,123 |
| Sample dn/dc [mL/g] | 0.137 |
| Sample IV [dL/g] | 0.236 |
Dextran is a typical polysaccharide and chromatographs very well. This dextran has a broad polydispersity index PDI = Mw/Mn value of 1.38. It is typically used as a system suitability sample for aqueous polymer applications, and often also a good choice for system calibration.
Previously
- Where can I find dn/dc?
- Biophysical Characterization: Questions and Answers
- 5 Steps to improve SEC-LS data