The Science of Beauty - Characterizing Hyaluronic Acid - the molecule behind anti-aging creams

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00:00:00 The Science of Beauty: Characterising Hyaluronic Acid - the molecule behind anti-aging creams
00:00:21 The Science of BeautyCharacterising Hyaluronic Acid – the molecule behind anti-aging creams
00:01:47 Agenda
00:02:46 Hyaluronic Acids
00:03:41 HA Chemistry
00:05:10 Samples analysed by GPC
00:06:22 HA Chemistry
00:08:03 Multi-detection GPC
00:09:19 Separation: SEC
00:10:33 Malvern Light Scattering range
00:11:37 SEC Instrument Schematic
00:12:29 Light Scattering Theory
00:13:53 Light Scattering Detector
00:14:35 Traditional Solution Viscosity Measurements
00:15:16 Viscometry
00:16:07 Traditional Solution Viscosity Measurements
00:18:46 Quantitative Comparison of Modified HA Structures
00:20:10 Quantitative Comparison of Modified HA Structures
00:21:05 The Kuhn–Mark–Houwink–Sakurada equation
00:22:06 Mark Houwink plot - Linear vs. Crosslinked
00:24:00 Structural Comparison of Modified HA Structures
00:25:22 Conformation plot - Linear vs. Crosslinked
00:27:05 Agenda
00:27:40 Critical overlap concentration (c*)
00:29:33 Determining values for c*
00:32:05 Coil overlap parameter c[η]
00:35:14 Dilute/semi-dilute behaviour c ≤ c*
00:38:28 Concentrated behaviour c > c*
00:41:20 Measuring polymer solutions using Microrheology
00:44:57 Brownian Motion, Particle Size and Viscosity
00:44:57 Mean Square Displacement
00:46:50 MSD Time Dependence on viscoelasticity
00:46:50 Extraction of rheological data
00:48:21 Extraction of rheological data
00:48:21 Different HA samples, same concentration
00:50:02 Different HA samples, same concentration
00:51:29 Same HA sample, different concentrations
00:52:12 Summary
00:53:04 Questions?
00:56:43 Contact Information

Hyaluronic acid (HA) is a natural polysaccharide, that plays an important role in tissues, such as skin, tendons, muscles, and cartilage. As well as medical applications, It finds wide-spread use in cosmetics, particularly skin creams. In this presentation, we demonstrate how a suite of combined analytical techniques can give insight into the structural difference that affect the efficacy of the final products. The information revealed includes molecular size and charge, absolute molecular weight distribution, branching structure and rheological properties.