Accuracy and reproducibility in automated dynamic light scattering measurements

In this application note, polystyrene latex standards are used to demonstrate the accuracy, reliability and reproducibility of the NanoSampler accessory for the Zetasizer Nano series of instruments. 60nm Polystyrene LTX3060A and 200nm beads LTX3200A [Duke Scientific, now Thermo Scientific] are prepared in filtered 64mM NaCl to obtain standard deviations of about 0.5% in batch, autosampled, as well as aliquot to aliquot experiments.The z-average cumulant size falls within the manufacturer stated range.


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