Biophysical characterization of viral and lipid-based vectors

Characterization of nucleic acid-based vaccines delivered as viral and non-viral vectors requires fit-for-purpose analytics and a significant level of method understanding In this peer-reviewed paper, you’ll discover how first-principle, label-free biophysical techniques can be applied to the characterization of the critical quality attributes of viruses and lipid nanoparticles.

You’ll learn:

  • How the combination of complementary label-free biophysical techniques have been successfully used for the characterization of physical and chemical attributes of rAAV and LNPs encapsulating mRNA
  • Explore analytical techniques, including dynamic light scattering (DLS), multiangle-DLS (MADLS), Electrophoretic Light Scattering (ELS), nanoparticle tracking analysis (NTA), multiple detection SEC and differential scanning calorimetry (DSC)
  • How to apply these techniques to measure multiple, critical quality attributes, including particle size distribution, aggregation propensity, polydispersity, particle concentration, particle structural properties and nucleic acid payload


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