Characterization of nucleic acid-based vaccines delivered as viral and non-viral vectors requires fit-for-purpose analytics and a significant level of method understanding In this peer-reviewed paper, you’ll discover how first-principle, label-free biophysical techniques can be applied to the characterization of the critical quality attributes of viruses and lipid nanoparticles.
You’ll learn:
- How the combination of complementary label-free biophysical techniques have been successfully used for the characterization of physical and chemical attributes of rAAV and LNPs encapsulating mRNA
- Explore analytical techniques, including dynamic light scattering (DLS), multiangle-DLS (MADLS), Electrophoretic Light Scattering (ELS), nanoparticle tracking analysis (NTA), multiple detection SEC and differential scanning calorimetry (DSC)
- How to apply these techniques to measure multiple, critical quality attributes, including particle size distribution, aggregation propensity, polydispersity, particle concentration, particle structural properties and nucleic acid payload
