Ultra Performance Liquid Chromatography (UPLC)

Multi-detector UPLC / UHPLC provides faster and more detailed analysis of polymer molecular weight, size and structure

Add multi-detection to your Waters ACQUITY APC™ or UHPLC system for faster and more detailed polymer analysis.

Do you have polydisperse polymers or oligomers and wish you were better able to resolve this distribution by size or molecular weight?  A clearer understanding of the molecular weight distribution might be the difference between a working or a failing polymer.  Or, would you like to do GPC/SEC measurements in half the time and using half the solvent (or less)?  Saving time and solvent means higher productivity, greener chemistry, lower operating costs, and a quicker turnaround from plant production to lab analysis.

Ultra high performance liquid chromatography (UHPLC) and ultra performance liquid chromatography (UPLC) such as the Waters ACQUITY® APC™ are an advancement on traditional gel permeation chromatography (GPC) separations.  Using advanced hybrid particles, which are smaller than in analytical GPC columns, ACQUITY APC columns offer improved resolutions, faster separation, reduced solvent use, and utilize low-dispersion (band broadening) hardware. A follow-on benefit is that the tall narrow peaks achieved using the ACQUITY APC help to improve sensitivity and reduce detection limits.


UPLC, or UHPLC (Ultra High Performance Liquid Chromatography) and HPLC are both liquid chromatography techniques to separate the components of a compound or mixture. The table below highlights some of the differences between these two separation techniques. 

Typical Column dimensions7.8 x 300 mm4.6 x 150 mm
Particle size3 - 10 µm< 3 µm
Typical Run time per injection45 minutes10 minutes
Solvent usage per injection45 mL10 mL
Pump pressure4 MPa70 MPa
Sample usage
(µg loading varies depending on sample and system set-up)
300 µg20 µg
Peak resolutionGoodBest
Mobile phase change overNormalFast

Adding multiple advanced detectors, including light scattering and viscometry to UPLC setups offers superior information about polymers and oligomers, including absolute molecular weight, size, and structure, when compared with conventional single-detector measurements.  Previously these two techniques have been incompatible due to band-broadening and dispersion effects that take place as narrow UPLC peaks pass through multiple detector cells. However, the latest enhancements to Malvern Panalytical's OMNISEC REVEAL detector platform now bring these two class leading technologies together.  Combining the information-rich data from advanced multi-detection with the speed and resolution afforded by UPLC it is now possible to get the best of both worlds - faster and more detailed polymer analysis.



Le système GPC/SEC multi-détecteurs le plus avancé au monde

Plus de détails
Plus de détails
Chromatographie par perméation de gel
Chromatographie d'exclusion de taille (SEC)
Diffusion statique de la lumière
Type de mesure
Structure moléculaire
Taille moléculaire
Masse molaire
Masse d'échantillon minimum (BSA) 100ng
Masse d'échantillon minimum (polystyrène) 100ng
Gamme de masse molaire 200Da - 10MDa