Get the best of both worlds: Coupling advanced multi-detection with UPLC for faster and more detailed polymer analysis

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00:00:00 Welcome
00:00:19 Mark Pothecary
00:00:25 Multi-detector UPLC
00:01:38 Size-exclusion chromatography
00:03:23 Overview of Multi-detector sec
00:03:34 Multi-detector SEC
00:05:08 Refractive index
00:06:05 UV-Vis Detection
00:07:06 Static light scattering
00:08:26 Intrinsic viscosity
00:09:46 Mark-Houwink plots
00:11:16 Branching
00:11:56 The multi-detection pyramid
00:13:08 Multi-detector SEC summary
00:13:29 Watersacquity APC
00:16:35 Waters ACQUITY APC
00:19:34 Multi-detector UPLCOMNISEC & APC
00:19:43 Why are we interested in UPLC?
00:21:10 Challenges involved with Multi-detection UPLC
00:22:18 Analytical GPC
00:22:56 Dispersion in UPLC
00:23:36 Hardware improvements
00:25:58 Dispersion in UPLC
00:26:58 Improve sensitivity
00:27:08 Sensitivity in analytical SEC
00:27:26 Sensitivity in UPLC
00:28:21 Improve measurement speed
00:28:29 Polystyrene Branching
00:29:57 PLA/PLGA
00:30:07 Copolymer PLGA 75:25
00:30:38 PLA/PLGA
00:31:16 Drug delivery polymer
00:32:57 Improve resolution
00:33:18 Oligomeric resolution
00:34:19 POlycaprolactone
00:34:24 PAO
00:36:41 Multi-detector UPLC
00:37:53 Thanks for listening
00:38:17 Contact Information

Multi-detector GPC/SEC offers superior information about polymers, including absolute molecular weight, size, and structure, when compared with conventional single-detector measurements. At the same time, UPLC offers a step-change in the resolution and speed of polymer separations, using small columns and particle sizes to achieve high plate counts. Previously these two techniques have been incompatible due to band-broadening and dispersion effects that take place as narrow UPLC peaks pass through multiple detector cells.The latest enhancements to Malvern Panalytical's OMNISEC REVEAL detector platform now bring these two class leading technologies together. Combining the information-rich data from multi-detector GPC/SEC with the speed and resolution afforded by UPLC (Water's ACQUITY APC).

This webinar will briefly describe advanced detection and UPLC, and discuss how these two technologies have been brought together. Application examples will be provided showing how this data really can be generated in just a few minutes.


Mark Pothecary studied biochemistry while an undergraduate at the University of Bath before moving to London to obtain his Masters and PhD the William Harvey Research Institute, part of the Queen Mary College between 2002-2006. There he studied the biochemical effects of red wine polyphenols on the production of nitric oxide in blood vessels and the subsequent control of blood pressure.

Mark joined Malvern in 2008 as a product technical specialist for the Zetasizer Nano and Viscotek products concentrating on bioscience SEC applications. He is now the Americas Product Manager for our chromatography products.

자세한 내용

Why attend?

Learn about the benefits of coupling Malvern Panlytical's OMNISEC REVEAL multi-dector to Water's ACQUITY APC, such as quicker measurements, reduced solvent and more detailed information about your macromolecules.

Who should attend?

Scientists looking to save time and money by upgrading to multi-detector UPLC, including;

  • GPC/SEC users
  • UPLC/UHPLC users
  • polymer chemists
  • biochemists
  • Lab managers
  • anyone interested in molecular weight and absolute molecular weight or polymer characterization

What will you learn?

The background of multi-detector GPC/SEC and UPLC measurements and how they can be combined to provide more detailed information about your macromolecules in a much shorter time.