Gel permeation / size exclusion chromatography (GPC/SEC) is the most well-known method for polymer and macromolecular analysis. As the technique has become more pervasive, there has been a push toward greater efficiency, by way of reduced run times and minimal solvent and sample consumption. The development of Advanced Polymer Chromatography (APC™) systems has accommodated these issues, by employing low volume columns and pumps with the ability to handle higher pressure generated by these columns. These features allowed for more efficient polymer analyses, but unfortunately, the technique still relies on the need for column calibration to provide relative molecular weight values. To determine if the efficiency of the ACQUITY APC unit and the power of the advanced detection of OMNISEC REVEAL were additive, two polymer samples with different structural features were analyzed. The goal of this experiment was to obtain enough characterization data to differentiate between linear and branched polystyrene samples – all in a matter of minutes.
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