Power in numbers - Learn more about your protein's behavior and stability in formulation by adding light scattering functionality to your SEC system

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00:00:00 Welcome
00:00:57 "Power in numbers" - Learn more about your protein's behaviour and stability in formulation by adding light scattering functionality to your SEC system.
00:03:22 Proteins
00:04:26 Size Exclusion Chromatography (SEC)
00:05:29 Conventional SEC
00:05:42 Conventional SEC
00:07:33 Beta-amylase: Conventional SEC
00:08:18 BSA: Column calibration
00:09:51 Limitation of available standards
00:11:07 Limitations of Conventional SEC
00:13:22 Light Scattering Detectors
00:13:58 Advanced Detection SEC
00:14:30 Malvern SEC range
00:15:12 How does Light scattering Give me Molecular Weight?
00:15:25 Light Scattering Theory
00:15:50 Light Scattering Theory
00:16:45 Isotropic Scatterers
00:18:14 Anisotropic Scatterers
00:20:06 Multi-Angle Light Scattering (MALS)
00:20:42 Multi-Angle Light Scattering (MALS)
00:22:23 For more information ……
00:22:52 SEC-mals application examples
00:23:01 Oligomerization Vs. Aggregation
00:23:36 BSA molecular weight using MALS
00:25:21 BSA measurements with MALS
00:26:08 BSA: Column calibration vs MALS
00:26:44 Pepsin aggregation
00:27:51 Pepsin aggregation
00:28:33 Immunoglobulin G
00:28:50 IgG molecular weight
00:30:04 IgG – How does sample loading effect the result?
00:31:01 Aprotinin
00:31:56 IgM
00:32:59 IgM - Lambda
00:33:51 IgM Lambda – Angular dependence
00:34:41 IgM – Kappa
00:36:01 Combined SLS & DLS
00:36:49 Introduction
00:38:14 Why Combine SLS and DLS?
00:39:37 Why combine SLS and DLS
00:41:32 Malvern Zetasizer µV
00:43:15 Multiple Analysis Modes
00:45:38 Application examples of zetasiZer µV
00:45:50 DLS in Batch Mode
00:47:55 DLS in Batch Mode
00:49:42 SLS in Batch Mode
00:51:05 Flow Mode SLS and DLS
00:53:14 Flow Mode SLS and DLS
00:55:14 Summary
00:57:36 Thank you
When developing a formulation stability profile for a biotherapeutics, one of the primary techniques used to identify the oligomeric state and presence or absence of aggregates is size exclusion chromatography (SEC). Traditionally, this technique provides a quantitative analysis of a sample by relating the retention volume of peaks present in the chromatogram to a calibration curve providing a basic identification of the relative molecular weight of each of the peaks.