Fecha registrada: June 07 2016

Duration: 25 minutes 08 seconds

• Nanoparticle Tracking Analysis (NTA) Characterization of Gold-Labeled Adeno Associated Virus (AAV) and Other Small Viruses 

• NTA is an analytical tool that measures the size and concentration of virus particles. 

• NTA can measure virus titer in about 5 minutes for viruses above Dh ~35 nm. 

• AAV (Dh ~ 25 nm) is too small and does not scatter enough light to be measured directly by NTA. 

• Gold-labeled AAV enhances the light scattering and enables NTA characterization to rapidly measure AAV particle concentration and aggregation state.

Table of contents
1. Welcome!
01:04
2. Contents
00:44
3. NanoSight Models
00:57
4. Adeno Virus (Directly From Serum)
01:51
5. Influenza Virus
01:02
6. Lentivirus
01:05
7. Small Viruses (35-45 nm)
00:59
8. Adeno Associated Virus (AAV)
03:24
9. AAV Titer
00:00
10. AAV Aggression State: Thermal Stress Test
01:38
11. AAV Electrostatic Gold Labeling Approach
00:52
12. Electrostatic vs. Antibody approach
01:17
13. TEM Analysis
00:00
14. Optical Arrangement for NanoSight Instruments
01:00
15. Direct Visualization of Nanoparticles in Suspension
00:00
16. Sizing: Stokes-Einstein
01:30
17. Concentration: Particles are Counted by Number
00:00
18. Fluorescent Mode
01:23
19. Analysis of 100 nm Fluorescence Standard Particles Suspended in FBS
00:00
20. Summary
01:36
21. Thank you for your attentionAny questions?
03:55
22. Contact Information
00:51