A basic introduction to Electrophoretic Light Scattering (ELS) for Zeta potential measurements

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00:00:00 Welcome
00:00:11 Introduction
00:00:41 A basic introduction to Electrophoretic Light Scattering (ELS) for Zeta potential measurements
00:00:46 Objectives
00:01:35 Dispersion Stability
00:08:28 DLVO Theory, 1940’s
00:11:48 Maintaining Dispersion Stability
00:13:42 ELECTROSTATICS:Origins of Surface Charge in Aqueous Media
00:13:55 Untitled
00:21:20 Factors Affecting Zeta Potential
00:21:40 Predicting Dispersion Stability
00:22:22 1.) Zeta Potential and pH
00:24:41 2.) Salt Ion - Conductivity
00:25:46 Non Specific Ion Adsorption
00:27:03 Specific Ion Adsorption
00:27:54 3.) Concentration of Surface Active Molecules
00:30:52 Electrokinetic Phenomena
00:30:57 ElectrokineticS
00:31:17 Electrokinetic Phenomena
00:31:47 Electrokinetic Phenomena
00:34:31 Untitled
00:34:41 Electrophoresis
00:35:08 Electrophoretic Light Scattering
00:35:49 Electrophoresis
00:36:14 Henry’s Function F(ka)
00:36:19 Henry’s Function F(ka)
00:36:39 Calculation of the Debye Length
00:36:55 Calculation of the Debye Length
00:37:36 Double Layer Thickness
00:38:14 Untitled
00:38:22 Optical Configuration of the Nano
00:39:46 Mixed Mode Measurement (M3)
00:40:57 Mixed Mode Measurement Phase Analysis Light Scattering (M3-PALS)
00:42:04 Voltage & Current Plot
00:43:04 FFR & SFR Phase Plot General Purpose Result
00:44:50 Conclusions
00:46:36 Thank you for attendingAsk your questions nowor email us at:ask@Malvernpanalytical.com

Electrophoretic Light Scattering (ELS) is a technique used to measure the electrophoretic mobility of particles or molecules in solution. This measurement is used to determine Zeta potential, which is a measure of the electrostatic or charge repulsion/attraction between particles, and is a predictor of emulsion and dispersion stability. Zeta potential is a function of both the particle surface and the dispersant. It provides information that is critical to formulators during the design of multicomponent products.

In this presentation Dr Ana Morfesis will discuss the importance of Zeta potential measurements for colloidal dispersions and how it is determined using ELS.


  • Dr Ana Morfesis 
    Ana is Applications Specialist for our Nanometrics product group. She joined Malvern Panalytical in 1999 and works with us in conjunction with the Carnegie Mellon University Lab. Ana has a PhD in Physical Chemistry from the University of Massachusetts. Her area of research has been colloid and surface chemistry since the early 1980s.

Pour en savoir plus

Who should attend?
Anyone who is working with or interested in Electrophoretic Light scattering or Zeta potential and would like an overview of the technique and its applications.

What will you learn?
How electrophoresis is measured and zeta potential calculated. What is the double layer, surface charge and interfacial characteristics as well as isoelectric point characterization.

Why attend?
This is an opportunity to get a basic overview of Electrophoretic Light Scattering and Zeta Potential, including key applications, from one of Malvern Panalytical's Zetasizer experts.