Antibody characterization in physiologically relevant conditions by grating-coupled interferometry (GCI)

Protein interaction analysis is crucial for the successful identification of drug targets. Amongst the wide range of biophysical techniques available today, surface-based biosensors such as SPR utilize optical systems to study biomolecular interactions without labels. Grating-Coupled Interferometry (GCI) is a novel label-free technique suitable for the analysis of low-molecular-mass drugs, multiprotein complexes and even larger particles like viruses or bacteria, with a remarkably wide affinity range (from mM to pM). Here, we show selected examples of GCI applications in the field of antibody characterization, including binding kinetics and affinity measurements in physiologically relevant media (serum, plasma, cell supernatant), that highlight how this technology is revolutionizing the study of molecular interactions and drug discovery.

Protein interaction analysis is crucial for the successful identification of drug targets. Amongst the wide range of biophysical techniques available today, surface-based biosensors such as SPR utilize optical systems to study biomolecular interactions without labels. Grating-Coupled Interferometry (GCI) is a novel label-free technique suitable for the analysis of low-molecular-mass drugs, multiprotein complexes and even larger particles like viruses or bacteria, with a remarkably wide affinity range (from mM to pM). Here, we show selected examples of GCI applications in the field of antibody characterization, including binding kinetics and affinity measurements in physiologically relevant media (serum, plasma, cell supernatant), that highlight how this technology is revolutionizing the study of molecular interactions and drug discovery.

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