Measuring Protein Charge: Historical Challenges & Recent Developments

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00:00:00 Measuring Protein Charge – Historical Challenges & Recent Developments
00:01:54 Protein Zeta Potential & Charge Historical Challenges & Recent Developments
00:02:52 Outline
00:03:32 Protein Charge – Increasing Interest
00:05:02 Google Scholar Citations
00:06:05 Importance
00:08:23 High Concentration Biotherapeutics
00:09:47 Pioneers
00:10:44 Protein Charge vs. pH
00:12:09 Not As “Simple” As It Might Seem
00:13:32 Calculating Protein Charge
00:16:34 Calculating Protein Charge – Methodology
00:17:17 Capillary Electrophoresis (CE)
00:18:39 CE Pros, And Cons
00:21:07 CE vs. pH Titration
00:23:42 Membrane Confined Electrophoresis (MCE)
00:24:44 MCE Pros And Cons
00:25:49 Electrophoretic Light Scattering (ELS)
00:27:30 ELS Pros And Cons
00:29:19 Invasive??
00:30:44 Protein ELS Measurements – Guidelines
00:34:03 Solutions Currently Under Development
00:35:17 Protein Denaturation At The Electrodes
00:35:56 Integration Of A Diffusion Barrier*
00:37:41 Discarding The Rules
00:39:37 Example Diffusion Barrier ELS Results
00:40:49 Acknowledgements
00:41:32 Questions?
00:44:56 Contact Information
Surface charge can be used as a means of predicting protein aggregation. One common technique for measuring protein charge is electrophoretic light scattering. Historical challenges with this method causing sample aggregation are addressed