Virus, Vaccine, and VLP characterization with NTA

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00:00:00 Welcome
00:03:01 Contents
00:04:00 NanoSight Instruments Provide:
00:05:48 Principle of measurement
00:05:51 Direct Visualization of Nanoparticles in Suspension
00:05:51 Optical Arrangement for NanoSight Instruments
00:08:06 Principle of Measurement
00:08:06 NTA Sizing is an Absolute Method
00:09:10 Nanoparticle Tracking Analysis
00:10:10 Particle Sizing in Action- Software Analysis
00:10:10 NTA Detection Limits
00:12:19 Examples
00:12:26 Example 1: Purified Influenza Virus
00:12:26 Example 2: Harvest Influenza – Prior to Purification
00:12:26 Example 3: Adeno Virus (Purified)
00:12:26 Example 4: Virus Like Particles
00:12:26 Example 5 – Protein Aggregation
00:12:26 Example 6- Protein Aggregation at 50 Degrees Celsius
00:12:57 Example 7: Bacterial Contamination
00:12:57 Types of Virus
00:13:13 Types of Virus
00:13:50 Virus Types Detectable with NanoSight
00:14:28 Virus Types Currently Below Detection Limit
00:14:59 ADDITIONAL PARAMETERS OF Nta analyses
00:15:04 True Size Distribution Profile
00:15:04 True Size Distribution Profile
00:15:26 Measurement of Viral Titer
00:16:20 Particles are Counted
00:16:20 Resolving Mixtures Through Scatter Intensity
00:18:33 Fluorescent Mode Available
00:19:36 Analysis in Complex Biological Media
00:19:36 Analysis in Complex Biological Media- Fluorescent Mode
00:19:36 Comparative Technologies
00:19:43 Plaque Assays
00:20:30 Correlation: NanoSight, plaque assay, qPCR
00:21:10 Electron Microscopy
00:21:35 Flow Cytometric Techniques
00:22:20 NTA is Proven on a Wide Range of Nanoparticles
00:22:56 Parameters Measured-Simultaneously, 'Real Time', Particle by Particle
00:23:28 Nanosight- NTA Devices Worldwide Mapping
00:24:06 Questions and Answers
00:45:37 Contact Information

During this webinar, we introduce the Nanoparticle Tracking Analysis (NTA) technique and discuss how it can provide a range of useful characterization information for virus, vaccine and virus-like particle (VLP) applications. More than a simple particle size analysis, the combination of particle concentration measurements and the fluorescence mode option can provide new insights into your samples. The number-based particle concentration can replace plaque assays for process control of virus concentration and gives direct feedback on VLP formation. Subunit vaccines and other protein based therapeutics can be closely monitored for aggregation, both the typical polydisperse size distributions and quantification of aggregates.