Fecha registrada: February 27 2014

Duration: 01 hours 03 minutes 54 seconds

When developing a formulation stability profile for a biotherapeutics, one of the primary techniques used to identify the oligomeric state and presence or absence of aggregates is size exclusion chromatography (SEC). Traditionally, this technique provides a quantitative analysis of a sample by relating the retention volume of peaks present in the chromatogram to a calibration curve providing a basic identification of the relative molecular weight of each of the peaks.
Table of contents
1. Welcome
00:57
2. "Power in numbers" - Learn more about your protein's behaviour and stability in formulation by adding light scattering functionality to your SEC system.
02:25
3. Proteins
01:04
4. Size Exclusion Chromatography (SEC)
01:03
5. Conventional SEC
00:13
6. Conventional SEC
01:51
7. Beta-amylase: Conventional SEC
00:45
8. BSA: Column calibration
01:33
9. Limitation of available standards
01:16
10. Limitations of Conventional SEC
02:15
11. Light Scattering Detectors
00:36
12. Advanced Detection SEC
00:32
13. Malvern SEC range
00:42
14. How does Light scattering Give me Molecular Weight?
00:13
15. Light Scattering Theory
00:25
16. Light Scattering Theory
00:55
17. Isotropic Scatterers
01:01
18. RIGHT-ANGLE LIGHT SCATTERING (RALS)
00:28
19. Anisotropic Scatterers
01:52
20. Multi-Angle Light Scattering (MALS)
00:36
21. Multi-Angle Light Scattering (MALS)
01:41
22. For more information ……
00:29
23. SEC-mals application examples
00:09
24. Oligomerization Vs. Aggregation
00:35
25. BSA molecular weight using MALS
01:45
26. BSA measurements with MALS
00:47
27. BSA: Column calibration vs MALS
00:36
28. Pepsin aggregation
01:07
29. Pepsin aggregation
00:42
30. Immunoglobulin G
00:17
31. IgG molecular weight
01:14
32. IgG – How does sample loading effect the result?
00:57
33. Aprotinin
00:55
34. IgM
01:03
35. IgM - Lambda
00:52
36. IgM Lambda – Angular dependence
00:50
37. IgM – Kappa
01:20
38. Combined SLS & DLS
00:48
39. Introduction
01:25
40. Why Combine SLS and DLS?
01:23
41. Why combine SLS and DLS
01:55
42. Malvern Zetasizer µV
01:43
43. Multiple Analysis Modes
02:23
44. Application examples of zetasiZer µV
00:12
45. DLS in Batch Mode
02:05
46. DLS in Batch Mode
01:47
47. SLS in Batch Mode
01:23
48. Flow Mode SLS and DLS
02:09
49. Flow Mode SLS and DLS
02:00
50. Summary
02:22
51. Thank you
06:18