| 00:00:00 | Understanding Size-exclusion Chromatography Basics: Choosing the Correct Columns and Detectors |
| 00:02:54 | Understanding Size-exclusion Chromatography Basics: Choosing the Correct Columns and Detectors |
| 00:04:31 | Outline |
| 00:05:52 | Defining Some Terms |
| 00:09:20 | Why Use Size-exclusion Chromatography? |
| 00:12:41 | Sample Solubility Required |
| 00:14:37 | Components of an SEC System |
| 00:15:48 | The Separation Process |
| 00:19:26 | Plate Theory |
| 00:22:06 | Flow-rate Effects – van-Deemter |
| 00:26:03 | SEC Chromatogram – Gaussian Peak |
| 00:28:09 | Deviation from Ideal Peak Shape |
| 00:30:38 | Column Range |
| 00:33:00 | Column Resolution |
| 00:35:05 | Calibration Slope |
| 00:37:53 | Selecting a GPC/SEC Column |
| 00:42:05 | Common Mobile Phases – General Guidelines |
| 00:42:56 | Why Are Salts and Buffers Used? |
| 00:44:50 | SEC Performance Factors Summary |
| 00:46:36 | SEC/GPC Data Processing Options |
| 00:48:23 | Column Calibration Configurations |
| 00:50:18 | Absolute Detection Configurations |
| 00:51:44 | Making the Final Decision |
| 00:53:47 | Always Remember |
| 00:55:16 | Conclusions |
| 00:56:45 | Defining Some Terms |
| 01:08:59 | Contact Information |
| 01:10:23 | Contact Information |
A presentation of the fundamentals of GPC/SEC, the key performance parameters and the data that can be obtained. Understanding these basic topics will enable the proper columns and chromatographic conditions to be chosen