| 00:00:00 | Speed vs. Detail: Dynamic light scattering (DLS) vs size exclusion chromatography (SEC) |
| 00:01:25 | Speed vs. Detail: Dynamic light scattering (DLS) vs size-exclusion chromatography (SEC) |
| 00:02:19 | Outline |
| 00:03:03 | Introduction to DLS and (multi-detector)-SEC |
| 00:03:11 | Dynamic Light Scattering |
| 00:06:08 | Size Data |
| 00:09:28 | Hardware for Analytical SEC |
| 00:11:02 | Multi-detector-SEC |
| 00:14:14 | So what? |
| 00:14:34 | What can DLS do? |
| 00:14:55 | Effect of Storage Conditions |
| 00:19:14 | Purity – Polydispersity |
| 00:20:36 | What can DLS do? |
| 00:20:55 | Protein stabilised by ligand binding |
| 00:23:18 | What can DLS do? |
| 00:23:44 | Protein Stability |
| 00:27:09 | What can DLS do? |
| 00:27:32 | Formulation |
| 00:30:23 | What can DLS do? |
| 00:31:01 | Limitations of DLS |
| 00:32:41 | What can SEC do? |
| 00:32:50 | Molecular weight measurements of BSA |
| 00:35:25 | Resolution |
| 00:37:20 | What can SEC do? |
| 00:37:31 | Quantification of antibody aggregates |
| 00:39:36 | What can SEC do? |
| 00:39:51 | Composition – Membrane proteins |
| 00:41:36 | Composition – Membrane proteins |
| 00:44:02 | PEGylated Protein |
| 00:44:39 | Conjugation analysis |
| 00:45:39 | What can SEC do? |
| 00:45:56 | Conformational changes |
| 00:49:02 | What can SEC do? |
| 00:49:16 | Limitations of SEC |
| 00:50:53 | Summary of DLS and SEC – pros and cons |
| 00:52:15 | Summary |
| 00:52:48 | So the real question is….. |
| 00:52:59 | So the real question is….. |
| 00:53:07 | Thanks for listening Any questions? |
| 00:53:22 | Contact details |
This webinar gives examples of the complementary nature of dynamic light scattering for fast aggregate detection and size exclusion chromatography for the composition and molecular weight characterization of proteins