NanoSight NS500 Zeta Potential Analysis Flow Chart for Use with NTA Software Versions 3.1 and 3.2

Introduction

The NanoSight NS500 instrument is able to generate high resolution particle-by-particle zeta potential measurements when fitted with the zeta potential option. Zeta potential measurements are derived at the same time as particle size data enabling users to have a direct verification of the impact of a particles' size to its zeta potential as well as the overall colloid stability of the sample.

The flow charts in this document provide a walk through of how to start and prime the fluidic systems of the NS500, find the correct measurement location, load a sample, generate and run a zeta potential script, flush and shut down the system.

Flowchart: Instructions for how to switch on the NanoSight NS500

Flowchart: Instructions for how to prime the fluidic system on the NanoSight NS500

Flowchart: Instructions for how load a sample using the on-board fluids on the NanoSight NS500

Flowchart: Instructions for how to set the measurement capture location and settings on the NanoSight NS500

Flowchart: Instructions for how to correctly set up the NanoSight NS500 prior to making a particle by particle zeta potential measurement

Flowchart: Instructions for how to set up a particle by particle zeta potential measurement script on the NanoSight NS500

Flowchart: Instructions for how to run a particle by particle zeta potential measurement script on the NanoSight NS500 including setting process settings and exporting data

Flowchart: Instructions for how to remove a sample from the NanoSight NS500 ready to measure further samples or to shut down the instrument

Flowchart: Instructions for how to shut down the NanoSight NS500

Introduction

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