Date d'enregistrement: March 15 2016

Duration: 35 minutes 58 seconds

There are many technologies available for the increased understanding of protein unfolding and stability, including calorimetry, spectroscopy, and light scattering techniques. In recent years, Raman spectroscopy has proven to be useful for the characterization of protein therapeutics, with its ability to monitor protein conformation via multiple secondary and tertiary structural markers acquired from a single measurement.

Additionally, Raman spectroscopy is perfectly suited for studying high concentration protein samples (as high as 150 mg/mL) with minimal, if any, sample preparation. By combining dynamic light scattering (DLS) and Raman spectroscopy onto a single platform, information about protein hydrodynamic size distributions and conformational changes can be obtained from a single sample under formulation conditions, leading to increased understanding of protein aggregation and unfolding pathways.

This webinar will introduce the combined platform of DLS and Raman spectroscopy and provide a case study of biotherapeutic relevance.
Table of contents
1. The Combination of Dynamic Light Scattering and Raman Spectroscopy for the Analysis of Protein Structure and Stability
01:13
2. Protein Aggregation and Stability
00:18
3. Untitled
00:46
4. Untitled
00:41
5. Untitled
00:20
6. Untitled
00:41
7. Untitled
00:42
8. Untitled
00:41
9. Untitled
00:30
10. Zetasizer Helix: Raman Spectra of a mAb
01:33
11. Zetasizer Helix: Data Acquisition Modes
00:52
12. Zetasizer Helix: Data Analysis Software
01:08
13. Application Areas
01:23
14. Case Study: Comparing the Thermal Stability and Aggregation Kinetics of a Biosimilar Formulation to the Innovator Product
00:20
15. Comparing the Thermal Stability and Aggregation Kinetics of a Biosimilar Formulation to the Innovator Product
01:12
16. Comparison of mAb A Formulation and mAb B Formulation at 20 °C
00:13
17. Comparing Spectral Data from mAb A and mAb B at 20 °C
00:26
18. Untitled
00:39
19. Untitled
00:08
20. Comparing DLS Data from mAb A and mAb B at 20 °C
00:28
21. Untitled
00:37
22. Thermal Ramp Measurements: Evaluating Thermal Stability at Formulation Conditions
00:10
23. Thermal Stability Measurement of mAb ASize and Conformation
00:55
24. Untitled
00:48
25. Untitled
00:15
26. Untitled
00:42
27. Untitled
00:45
28. Untitled
00:16
29. Thermal Stability Summary and Comparison of mAb A and mAb B
01:44
30. Untitled
00:46
31. Isothermal Incubation Measurements: Correlating Aggregation Kinetics and Structural Change
00:29
32. Isotherm Incubation Studies Near Melting/Transition Temperature
01:27
33. Untitled
01:02
34. Isotherm Incubation Studies Near Melting/Transition Temperature
01:26
35. Isotherm Incubation Studies Near Melting/Transition Temperature
01:53
36. Isotherm Incubation Studies Near Melting/Transition Temperature
01:41
37. Isothermal Incubation Summary and Conclusions
01:03
38. Case Study Summary
00:26
39. Untitled
00:11
40. Thank you for your attentionAny questions?
00:12
41. Isotherm Incubation Studies Near Melting/Transition Temperature
00:53
42. Thank you for your attentionAny questions?
00:39
43. Untitled
01:28
44. Thank you for your attentionAny questions?
01:09
45. Contact Information
00:47