Determining Steric Layer Thickness and Conformation Using Dynamic Light Scattering

Dynamic Light Scattering proves an excellent technique for making measurements of the thickness of an adsorbed Dextran layer used to enhance the stability of the casein stabilized emulsions used in food, cosmetics and pharmaceuticals.

Layer thickness varied from 2nm for unconjugated beta lactoglobulin up to 22nm for protein complexed with 440kDa dextrane. Asymptotic behavior indicated complete surface coverage.Surface layer increase is slightly larger than expected hydrodynamic diameter of the dextrane part, likely due to fnon-sphericity and surface orientation or dextran confirmation change on binding to beta lactoglobulin.

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