| 00:00:00 | Size-exclusion chromatography in the modern bio-pharmaceutical laboratory |
| 00:02:09 | Size-exclusion chromatography in the modern bio-pharmaceutical laboratory Uses, requirements, technologies and applications |
| 00:02:52 | Protein therapeutics |
| 00:04:21 | Pharmaceutical Drug Development Pipeline |
| 00:05:59 | Protein Characterisation |
| 00:07:10 | Current uses of SEC - analytical |
| 00:10:21 | Current uses of SEC - QC |
| 00:11:59 | Multi-detector Analytical SEC |
| 00:12:57 | UV Detection |
| 00:13:23 | RI Detection |
| 00:14:21 | Static Light Scattering – Molecular Weight |
| 00:15:20 | Intrinsic Viscosity |
| 00:16:35 | Analytical SEC Chromatogram |
| 00:17:52 | Advanced analytical SEC |
| 00:19:10 | GPC in QC (qGPC) |
| 00:19:43 | Protein Aggregation |
| 00:20:20 | Oligomerization vs. aggregation |
| 00:22:09 | Aggregated BSA experiments |
| 00:22:56 | Aggregated BSA experiments – SEC LS |
| 00:25:19 | Aggregated BSA experiments |
| 00:28:01 | Qualitative aggregate detection |
| 00:29:12 | Current uses of SEC in QC |
| 00:29:37 | What more information can be collected? |
| 00:30:03 | What data can be provided? |
| 00:34:37 | Therapeutic protein sample |
| 00:36:04 | Analytical SEC |
| 00:36:34 | Molecular weight and oligomeric state |
| 00:37:15 | Conformational changes |
| 00:40:09 | Case Study – Membrane Protein |
| 00:41:44 | Membrane Protein |
| 00:43:31 | Drug Delivery Polymer |
| 00:45:14 | Summary |
| 00:48:39 | Contact Information |
This presentation describes the positioning and usage of SEC as a technique in the bio-pharmaceutical industry. Common uses, regulatory requirements and standards, and the latest detector technology are discussed along with application examples.