Characterization of the Size and Molecular Weight and Oligomeric State of Proteins using the Malvern Zetasizer Systems in batch and flow modes

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00:00:00 Characterization of the Size and Molecular Weight and Oligomeric State of Proteins using the Malvern Zetasizer Systems in batch and flow modes
00:01:47 Characterization of the size, molecular weight and oligomeric state of proteins using the Malvern Zetasizer systems in batch and flow modes
00:02:51 Abstract
00:03:01 Contents
00:04:11 Introduction to Theory and Instruments
00:04:17 Introduction
00:05:31 Introduction
00:06:49 Introduction
00:08:31 Introduction
00:09:45 Introduction
00:10:59 Introduction
00:11:56 Electrophoretic Light Scattering
00:13:10 Comparison of Zetasizer Models
00:13:31 Malvern Zetasizer Nano ZS90
00:14:49 Malvern Zetasizer Nano ZS
00:16:32 Nano Zetasizer Nano ZSP
00:17:34 Malvern Zetasizer µV
00:18:54 Malvern Zetasizer APS
00:20:17 Model Summary
00:21:13 Example Results
00:21:30 The Proteins being Studied
00:23:51 Protein Analysis in Batch Mode
00:24:15 DLS in Batch Mode
00:25:13 DLS in Batch Mode
00:27:02 DLS in Batch Mode
00:28:25 DLS in Batch Mode
00:29:50 DLS in Batch Mode
00:30:23 SLS in Batch Mode
00:30:51 SLS in Batch Mode
00:31:19 SLS in Batch Mode
00:32:34 Protein Analysis in Flow Mode
00:33:48 Flow Mode SLS and DLS
00:35:11 Flow Mode SLS and DLS
00:36:48 Flow Mode SLS and DLS
00:37:48 Flow Mode SLS and DLS
00:38:17 Flow Mode SLS and DLS
00:39:01 Flow Mode SLS and DLS
00:39:30 Electrophoretic Light Scattering
00:40:42 Human Serum Albumin mobility at pH 7
00:41:45 Human Serum Albumin mobility at pH 4
00:42:40 BSA pH trend
00:43:01 Microrheology
00:44:08 Microrheology applications – protein solution viscosity
00:44:55 Microrheology applications – protein solution viscosity
00:45:36 Microrheology applications – protein aggregation
00:46:45 Model Summary
00:47:36 Questions and Further Information
00:51:13 Contact Information
This presentation will discuss the use of the Zetasizer Nano and Zetasizer µV systems for the measurement of the size, molecular weight and oligomeric state of proteins in solution, and the use of these systems as chromatography detectors