Fecha registrada: November 15 2016

Duration: 38 minutes 13 seconds

The self-association characteristics of molecules in dilute solutions can provide an assessment of stability at an early stage in development. The diffusion interaction parameter (kD) is a measure of the propensity for self-association, but the determination of this parameter using existing methodologies requires several measurements over a concentration series. Here, we show how Taylor Dispersion Analysis can be used to generate a concentration gradient from which the kD can be extracted in a single, low volume measurement, making this an excellent choice of technology for early stability assessment, particularly for biopharmaceuticals.
Table of contents
1. Welcome
00:13
2. Introduction
00:48
3. Using Taylor Dispersion Analysis for early stability assessment via a single kD measurement
00:19
4. Overview
00:32
5. Overview
00:04
6. Protein Stability and Aggregation
01:00
7. Protein Stability and Aggregation
00:45
8. Protein Stability and Aggregation
00:56
9. Measuring Protein Stability
01:29
10. Measuring Protein Stability
01:21
11. Characterisation across the pipeline
01:55
12. Measuring Protein Stability
00:40
13. What is kD and B22?
01:49
14. What is kD and B22?
01:09
15. Understanding kD and B22: Attractive and Repulsive Interactions
01:18
16. Understanding kD and B22: Attractive and Repulsive Interactions
00:43
17. Viscosity as an early predictor
00:40
18. Viscosity as an early predictor
00:31
19. Overview
00:20
20. Taylor Dispersion Analysis
01:03
21. Taylor Dispersion Analysis
00:38
22. Taylor Dispersion Analysis – Measuring Diffusion Coefficient
01:08
23. Taylor Dispersion Analysis – Measuring Hydrodynamic Radius
01:50
24. Taylor Dispersion Analysis – Frontal Injection
00:52
25. Taylor Dispersion Analysis – Measuring kD
01:26
26. Overview
00:13
27. Challenges of Peptide Characterisation
01:00
28. Selected Peptides
01:02
29. Peptide Characterisation
01:15
30. Comparison of the three Peptides based on kD
01:00
31. Formulation study of Bradykinin
00:57
32. Literature example: Peptide Characterisation using TDA
00:36
33. Literature example: Early developability screen of mAbs
01:42
34. Literature example: Early developability screen of mAbs
01:00
35. Conclusions
00:54
36. Thank you for your attentionAny questions?
04:21
37. Contact Information
00:44