| 00:00:00 | Welcome! |
| 00:01:04 | Contents |
| 00:01:48 | NanoSight Models |
| 00:02:45 | Adeno Virus (Directly From Serum) |
| 00:04:36 | Influenza Virus |
| 00:05:38 | Lentivirus |
| 00:06:43 | Small Viruses (35-45 nm) |
| 00:07:42 | Adeno Associated Virus (AAV) |
| 00:11:06 | AAV Titer |
| 00:11:06 | AAV Aggression State: Thermal Stress Test |
| 00:12:44 | AAV Electrostatic Gold Labeling Approach |
| 00:13:36 | Electrostatic vs. Antibody approach |
| 00:14:53 | TEM Analysis |
| 00:14:53 | Optical Arrangement for NanoSight Instruments |
| 00:15:53 | Direct Visualization of Nanoparticles in Suspension |
| 00:15:53 | Sizing: Stokes-Einstein |
| 00:17:23 | Concentration: Particles are Counted by Number |
| 00:17:23 | Fluorescent Mode |
| 00:18:46 | Analysis of 100 nm Fluorescence Standard Particles Suspended in FBS |
| 00:18:46 | Summary |
| 00:20:22 | Thank you for your attentionAny questions? |
| 00:24:17 | Contact Information |
• Nanoparticle Tracking Analysis (NTA) Characterization of Gold-Labeled Adeno Associated Virus (AAV) and Other Small Viruses
• NTA is an analytical tool that measures the size and concentration of virus particles.
• NTA can measure virus titer in about 5 minutes for viruses above Dh ~35 nm.
• AAV (Dh ~ 25 nm) is too small and does not scatter enough light to be measured directly by NTA.
• Gold-labeled AAV enhances the light scattering and enables NTA characterization to rapidly measure AAV particle concentration and aggregation state.