| 00:00:00 | Measuring Protein Charge – Historical Challenges & Recent Developments |
| 00:01:54 | Protein Zeta Potential & Charge Historical Challenges & Recent Developments |
| 00:02:52 | Outline |
| 00:03:32 | Protein Charge – Increasing Interest |
| 00:05:02 | Google Scholar Citations |
| 00:06:05 | Importance |
| 00:08:23 | High Concentration Biotherapeutics |
| 00:09:47 | Pioneers |
| 00:10:44 | Protein Charge vs. pH |
| 00:12:09 | Not As “Simple” As It Might Seem |
| 00:13:32 | Calculating Protein Charge |
| 00:16:34 | Calculating Protein Charge – Methodology |
| 00:17:17 | Capillary Electrophoresis (CE) |
| 00:18:39 | CE Pros, And Cons |
| 00:21:07 | CE vs. pH Titration |
| 00:23:42 | Membrane Confined Electrophoresis (MCE) |
| 00:24:44 | MCE Pros And Cons |
| 00:25:49 | Electrophoretic Light Scattering (ELS) |
| 00:27:30 | ELS Pros And Cons |
| 00:29:19 | Invasive?? |
| 00:30:44 | Protein ELS Measurements – Guidelines |
| 00:34:03 | Solutions Currently Under Development |
| 00:35:17 | Protein Denaturation At The Electrodes |
| 00:35:56 | Integration Of A Diffusion Barrier* |
| 00:37:41 | Discarding The Rules |
| 00:39:37 | Example Diffusion Barrier ELS Results |
| 00:40:49 | Acknowledgements |
| 00:41:32 | Questions? |
| 00:44:56 | Contact Information |
Surface charge can be used as a means of predicting protein aggregation. One common technique for measuring protein charge is electrophoretic light scattering. Historical challenges with this method causing sample aggregation are addressed