Date d'enregistrement: April 14 2010

Duration: 35 minutes 28 seconds

A presentation of how protein stability can be monitored using light scattering. Typical examples of applications such as detection of aggregates in protein formulations and the study of thermal or chemical stability of proteins will be given
Table of contents
1. How to monitor protein stability using light scattering techniques
02:40
2. How to monitor protein stability using light scattering techniques
00:10
3. Chemical and structural integrity of proteins
00:43
4. Therapeutic protein product stability
00:29
5. Protein Aggregation – Why The Concern?
01:11
6. Dynamic Light Scattering
01:17
7. What is measured with DLS – hydrodynamic size
01:27
8. Parameters that are used in the following SlideData
00:46
9. Light scattering is sensitive to size
00:49
10. Case studies
00:22
11. Stability study of Recombinant Human Serum Albumins
00:41
12. Comparison Albucult™ and Recombumin™
02:12
13. Electrostatic/Virial effect at high protein concentration
01:57
14. Polydispersity Index
00:46
15. Correlogram of low concentration samples of Recombumin
00:35
16. Comparison of size distributions at low concentrations
00:28
17. Recombumin™ in PBS
00:53
18. Albucult™ thermal trends
00:48
19. Protein peak size during thermal trend
00:56
20. Recombumin™ thermal trends
00:39
21. Recombumin™ thermal trends in different buffers
00:56
22. Recombumin™ formulation buffer on its own
00:37
23. pH stability test recombinant human serum albumin
00:44
24. DLS results show similar trend as GP.HPLC results
00:36
25. Summary Recombumin™ & Albucult™ samples
00:39
26. Hemoglobin based oxygen carriers (HBOCs)
01:14
27. Globin calibration curve-to estimate molecular weight
01:30
28. Globin based-calibration curve:
00:34
29. PEGylated hemoglobin data
01:39
30. Modification of proteins: PEGylated hemoglobins
01:20
31. Summary HBOCs
00:28
32. Dynamic light scattering and protein stability
00:46
33. Thank you!
00:33
34. Comparison of size distributions at low concentrations
01:41
35. Comparison Albucult™ and Recombumin™
01:17
36. Contact Information
01:05